ksgA [2020-04-29 15:43:32]

rRNA adenine dimethyltransferase

locus: BSU_00420

pI: 5.71

mw: 32.57 kDa

protein length: 292 aa

gene length: 879 bp

function: rRNA maturation

product: rRNA adenine dimethyltransferase

essential: no

ec: 2.1.1.-

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 50,640 → 51,518

The protein

Catalyzed reaction/ biological activity

• adenosine¹⁵¹⁸/adenosine¹⁵¹⁹ in 16S rRNA + 4 S-adenosyl-L-methionine --> 4 H⁺ + N⁶-dimethyladenosine¹⁵¹⁸/N⁶-dimethyladenosine¹⁵¹⁹ in 16S rRNA + 4 S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)
• [SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

Structure

• [PDB|6IFT] (complexed with S-adenosylmethionine) [Pubmed|30624914]
• [PDB|6IFS] [Pubmed|30624914]
• [PDB|6IFV] (C-terminal truncation) [Pubmed|30624914]
• [PDB|6IFW] (Chimeric protein) [Pubmed|30624914]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]-[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]
  description: [Pubmed|11233981]

Biological materials

Mutant

• MGNA-B907 (ksgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1906 NBRP B. subtilis, Japan]
• BKE00420 (Δ[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG
• BKK00420 (Δ[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG

References

• 16497325,19001112,11233981,19285505,30624914